Hotspot

psbK-psbI

LSC · 7574-7985 · 412 bp

Back to Erythrostemon

Pi 0.0385

MarkerSeek score 67.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 412
variable_sites: 15
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	ACGCCCCATTTCTATTCGAC	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	1337-1414 bp	1.0	84.8
psbK-psbI_p2	CACGCCCCATTTCTATTCGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	1338-1415 bp	1.0	84.8
psbK-psbI_p3	CGCCCCATTTCTATTCGACA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	1336-1413 bp	1.0	84.8
psbK-psbI_p4	ACGCCCCATTTCTATTCGAC	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	1338-1415 bp	1.0	84.8
psbK-psbI_p5	CGCCCCATTTCTATTCGACA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	1337-1414 bp	1.0	84.8