Hotspot

rps16-trnQ-UUG

LSC · 6209-6843 · 635 bp

Back to Erythrostemon

Pi 0.039

MarkerSeek score 66.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 635
variable_sites: 15
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GTCGAATAGAAATGGGGCGT	50.0	50.0	58.0	58.1	528-775 bp	1.0	85.4
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	TCGAATAGAAATGGGGCGTG	50.0	50.0	58.0	58.1	527-774 bp	1.0	85.4
rps16-trnQ-UUG_p3	GTCTTTCAAGTCGCACGTTG	TGTCGAATAGAAATGGGGCG	50.0	50.0	58.0	58.1	529-776 bp	1.0	85.4
rps16-trnQ-UUG_p4	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	468-715 bp	1.0	85.4
rps16-trnQ-UUG_p5	ATGTCTTTCAAGTCGCACGT	GTCGAATAGAAATGGGGCGT	45.0	50.0	58.1	58.1	530-777 bp	1.0	84.4