Hotspot

trnK-UUU-rps16

LSC · 4360-5066 · 707 bp

Back to Erythrostemon

Pi 0.05
MarkerSeek score 79.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
707
variable_sites
34
pi
0.05
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TGGAACTTTGTCCTAATCAACA 55.0 36.4 57.9 55.6 830-839 bp 1.0 49.7
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC TGGAACTTTGTCCTAATCAACAA 55.0 34.8 57.9 56.3 830-839 bp 1.0 47.6
trnK-UUU-rps16_p3 TGGAGTCGCACTTAAAAGCC TGGAACTTTGTCCTAATCAACA 50.0 36.4 58.5 55.6 784-793 bp 1.0 46.9
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG TGGAACTTTGTCCTAATCAACA 50.0 36.4 58.6 55.6 781-790 bp 1.0 46.2
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC ATGGAACTTTGTCCTAATCAACA 55.0 34.8 57.9 56.1 831-840 bp 1.0 46.0