Hotspot

trnH-GUG-psbA

LSC · 79-421 · 343 bp

Back to Erythrostemon

Pi 0.0712
MarkerSeek score 75.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
343
variable_sites
23
pi
0.0712
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TGTGGTTGAGGCTCCATCTA 55.0 50.0 58.1 58.0 395-424 bp 1.0 87.0
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CCCTCTAGACCTAGCTGTGG 55.0 60.0 58.1 58.3 410-439 bp 1.0 84.7
trnH-GUG-psbA_p3 GATCCACTTGGCTACATCCG CCGAGCTAACCTTGGTATGG 55.0 55.0 58.1 57.8 461-490 bp 1.0 84.3
trnH-GUG-psbA_p4 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGTGGT 55.0 55.0 58.1 57.6 409-438 bp 1.0 83.5
trnH-GUG-psbA_p5 CCTTGATCCACTTGGCTACA TGTGGTTGAGGCTCCATCTA 50.0 50.0 57.2 58.0 399-428 bp 1.0 81.6