Hotspot

ndhF

SSC · 115673-117913 · 2241 bp

Back to Erythrophleum

Pi 0.0055

MarkerSeek score 46.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 23
pi: 0.0055
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGATCTATTATGCGCCTTTGC	TGACCCATTTTCACTTCGTACT	47.6	40.9	57.9	57.7	2999 bp	0.25	22.4
ndhF_p2	TGTTTCGATGCAACAACCAA	TGACCCATTTTCACTTCGTACT	40.0	40.9	56.8	57.7	2750 bp	0.25	21.4
ndhF_p3	CGATCTATTATGCGCCTTTGC	TTGACCCATTTTCACTTCGT	47.6	40.0	57.9	55.5	3000 bp	0.25	21.0
ndhF_p4	TCTAGCATTGGGTAGACCTCA	TGACCCATTTTCACTTCGTACT	47.6	40.9	57.6	57.7	2977 bp	0.25	20.0
ndhF_p5	TGTTTCGATGCAACAACCAA	TTGACCCATTTTCACTTCGT	40.0	40.0	56.8	55.5	2751 bp	0.25	20.0