Hotspot

ycf1-ndhF

SSC · 115596-115672 · 77 bp

Back to Erythrophleum

Pi 0.165

MarkerSeek score 77.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 77
variable_sites: 22
pi: 0.165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GCTCGGGTTATGGAAGAAGG	GAATTCCAAATGGGGTGGGT	55.0	50.0	58.0	58.0	790-1127 bp	1.0	83.6
ycf1-ndhF_p2	GCTCGGGTTATGGAAGAAGG	TGGAATTCCAAATGGGGTGG	55.0	50.0	58.0	58.1	792-1129 bp	1.0	83.6
ycf1-ndhF_p3	GCTCGGGTTATGGAAGAAGG	TAAAATATGCAGGGGGTGGC	55.0	50.0	58.0	57.9	742-1079 bp	1.0	83.6
ycf1-ndhF_p4	GGGCCCTCTTATCTCTTCCT	GAATTCCAAATGGGGTGGGT	55.0	50.0	57.9	58.0	817-1154 bp	1.0	83.0
ycf1-ndhF_p5	AGGGCCCTCTTATCTCTTCC	GAATTCCAAATGGGGTGGGT	55.0	50.0	57.9	58.0	818-1155 bp	1.0	82.9