Hotspot

rps3-rps19

LSC · 88656-89340 · 685 bp

Back to Erythrophleum

Pi 0.0327

MarkerSeek score 74.60

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 39
pi: 0.0327
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	ACTCTAAACTTCCGGGGACA	50.0	50.0	57.5	58.0	776-935 bp	1.0	84.0
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	ACTCTAAACTTCCGGGGACA	50.0	50.0	58.6	58.0	770-929 bp	1.0	83.1
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	ACTCTAAACTTCCGGGGACA	45.0	50.0	57.0	58.0	814-973 bp	1.0	79.8
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	ACTCTAAACTTCCGGGGACA	45.0	50.0	57.0	58.0	769-928 bp	1.0	79.3
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	CTAAACTTCCGGGGACATGC	50.0	55.0	57.5	58.6	773-932 bp	1.0	79.2