Hotspot

accD-psaI

LSC · 63595-64003 · 409 bp

Back to Erythrophleum

Pi 0.0338

MarkerSeek score 66.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 20
pi: 0.0338
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTGATTCAATCGTACCGCGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	562-634 bp	1.0	86.9
accD-psaI_p2	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	666-738 bp	1.0	86.4
accD-psaI_p3	TTGATTCAATCGTACCGCGT	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	852-924 bp	1.0	85.9
accD-psaI_p4	TTGATTCAATCGTACCGCGT	ATACGTTCTGATCGCCAACC	45.0	50.0	58.0	58.1	817-889 bp	1.0	85.8
accD-psaI_p5	TGCTTACATTGCATTTGCGG	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	956-1028 bp	1.0	85.4