Hotspot

rbcL-accD

LSC · 61095-62094 · 1000 bp

Back to Erythrophleum

Pi 0.0269

MarkerSeek score 80.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 48
pi: 0.0269
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	CGGGTCTTCACTTACACTGG	50.0	55.0	58.0	57.9	1195-1272 bp	1.0	84.7
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	CGGGTCTTCACTTACACTGG	55.0	55.0	58.0	57.9	1210-1287 bp	1.0	84.6
rbcL-accD_p3	TTCGTGAGGCTAGCAAATGG	CGGGTCTTCACTTACACTGG	50.0	55.0	58.3	57.9	1215-1292 bp	1.0	82.8
rbcL-accD_p4	GGCTAGCAAATGGAGTCCTG	CGGGTCTTCACTTACACTGG	55.0	55.0	58.3	57.9	1208-1285 bp	1.0	82.3
rbcL-accD_p5	GTGAGGCTAGCAAATGGAGT	CGGGTCTTCACTTACACTGG	50.0	55.0	57.6	57.9	1212-1289 bp	1.0	81.6