Hotspot

ndhC-trnV-UAC

LSC · 55126-55795 · 670 bp

Back to Erythrophleum

Pi 0.0226
MarkerSeek score 69.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
670
variable_sites
23
pi
0.0226
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.86
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGCTAATCGGGGCTAA AAAAGATGCACGTTGGGTCT 50.0 45.0 57.7 58.0 794-884 bp 1.0 83.3
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA TGCACGTTGGGTCTGTAAAA 50.0 45.0 57.7 58.0 788-878 bp 1.0 83.2
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 723-813 bp 1.0 82.1
ndhC-trnV-UAC_p4 CCTTTGCTAATCGGGGCTAA GATGCACGTTGGGTCTGTAA 50.0 50.0 57.7 58.2 790-880 bp 1.0 81.9
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA ATGCACGTTGGGTCTGTAAA 50.0 45.0 57.7 57.7 789-879 bp 1.0 81.4