Hotspot

psbZ-trnG-GCC

LSC · 39760-40288 · 529 bp

Back to Erythrophleum

Pi 0.014

MarkerSeek score 70.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 529
variable_sites: 13
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	666-684 bp	1.0	81.0
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	669-687 bp	1.0	79.4
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	662-680 bp	1.0	79.3
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	667-685 bp	1.0	79.0
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCGA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	58.4	57.7	665-683 bp	1.0	78.2