Hotspot

trnS-GCU-trnG-UCC

LSC · 9276-10040 · 765 bp

Back to Erythrophleum

Pi 0.0379

MarkerSeek score 82.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 48
pi: 0.0379
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	813-877 bp	1.0	82.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	812-876 bp	1.0	82.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.2	57.3	867-931 bp	1.0	81.4
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	781-845 bp	1.0	80.5
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	780-844 bp	1.0	80.5