Hotspot

trnQ-UUG-psbK

LSC · 7703-8301 · 599 bp

Back to Erythrophleum

Pi 0.0248

MarkerSeek score 74.40

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 599
variable_sites: 26
pi: 0.0248
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCGCG	55.0	50.0	57.6	58.6	719-767 bp	1.0	78.2
trnQ-UUG-psbK_p2	GATATGCTCTGGGACGGAAG	GGCTTCAGGTAATTTCGCGA	55.0	50.0	57.6	58.6	718-766 bp	1.0	78.2
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	58.7	58.6	677-725 bp	1.0	76.5
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	58.7	58.6	676-724 bp	1.0	76.5
trnQ-UUG-psbK_p5	AATGGATATGCTCTGGGACG	AGGCTTCAGGTAATTTCGCG	50.0	50.0	57.2	58.6	723-771 bp	1.0	75.0