Hotspot

trnK-UUU-rps16

LSC · 4419-5775 · 1357 bp

Back to Erythrophleum

Pi 0.0194
MarkerSeek score 66.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1357
variable_sites
44
pi
0.0194
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.80
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1312-1584 bp 1.0 85.6
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC CCTTGAAAAAGGCGCTCAAC 55.0 50.0 57.9 58.2 1317-1589 bp 1.0 84.0
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC CTTGAAAAAGGCGCTCAACC 55.0 50.0 57.9 58.2 1316-1588 bp 1.0 84.0
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TGAAAAAGGCGCTCAACCTA 55.0 45.0 57.9 57.7 1314-1586 bp 1.0 83.7
trnK-UUU-rps16_p5 CCGATGGTATGGACGAATCC TCCTTGAAAAAGGCGCTCAA 55.0 45.0 57.9 58.3 1318-1590 bp 1.0 83.3