Hotspot

ycf1

SSC · 121787-127174 · 5388 bp

Back to Erythronium

Pi 0.0145

MarkerSeek score 51.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5388
variable_sites: 249
pi: 0.0145
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTCTCTTCTTTATCAAAATTGGA	CGTGTTCAAAGACGCAAAAC	29.2	45.0	54.5	56.8	180-189 bp	1.0	51.2
ycf1_p2	TGTCTCTTCTTTATCAAAATTGGA	CGTGTTCAAAGACGCAAAACA	29.2	42.9	54.5	58.5	180-189 bp	1.0	51.2
ycf1_p3	TGTCTCTTCTTTATCAAAATTGGAA	CGTGTTCAAAGACGCAAAAC	28.0	45.0	55.2	56.8	180-189 bp	1.0	51.2
ycf1_p4	TTGTCTCTTCTTTATCAAAATTGGA	CGTGTTCAAAGACGCAAAAC	28.0	45.0	55.2	56.8	181-190 bp	1.0	51.1
ycf1_p5	TGTTCTTTTGTCTCTTCTTTATCA	CGTGTTCAAAGACGCAAAAC	29.2	45.0	54.4	56.8	188-197 bp	0.909091	46.7