Hotspot

rpoA

LSC · 76692-77711 · 1020 bp

Back to Erythronium

Pi 0.00613

MarkerSeek score 37.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 21
pi: 0.00613
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GTGGGCTTAGCGGTTTTAGA	GTAGTTGATCAGGGCATGCA	50.0	50.0	57.9	58.0	1858-1871 bp	1.0	81.1
rpoA_p2	GTGGGCTTAGCGGTTTTAGA	TACGAGCCATCCGTAGAAGT	50.0	50.0	57.9	58.0	1788-1801 bp	1.0	81.1
rpoA_p3	GTGGGCTTAGCGGTTTTAGA	GCGATGTAACACCTATGCCA	50.0	50.0	57.9	58.1	1746-1759 bp	1.0	81.1
rpoA_p4	CGTCGTCCAGTAGCTACAAC	TACGAGCCATCCGTAGAAGT	55.0	50.0	58.1	58.0	1569-1582 bp	1.0	81.1
rpoA_p5	CGTCGTCCAGTAGCTACAAC	GTAGTTGATCAGGGCATGCA	55.0	50.0	58.1	58.0	1639-1652 bp	1.0	81.0