Hotspot

rps15-ycf1

SSC · 121371-121786 · 416 bp

Back to Erythronium

Pi 0.0255

MarkerSeek score 60.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 416
variable_sites: 37
pi: 0.0255
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCATTGATGGGTGATGAGG	ACCCATAACAGCAGTCGTTT	50.0	45.0	57.9	57.4	921-984 bp	1.0	80.6
rps15-ycf1_p2	TTCCCAATTGTTCTGGTGCA	ACCCATAACAGCAGTCGTTT	45.0	45.0	57.9	57.4	1124-1187 bp	1.0	79.8
rps15-ycf1_p3	AACACCATGCATTGATGGGT	ACCCATAACAGCAGTCGTTT	45.0	45.0	58.1	57.4	928-991 bp	0.909091	76.7
rps15-ycf1_p4	TGCATTGATGGGTGATGAGG	TTCTTTGGCCCAATTACCGA	50.0	45.0	57.9	57.4	980-1032 bp	0.909091	76.5
rps15-ycf1_p5	AACACCATGCATTGATGGGT	TTCTTTGGCCCAATTACCGA	45.0	45.0	58.1	57.4	987-994 bp	0.818182	72.6