Hotspot

rpl32-trnL-UAG

SSC · 111483-112162 · 680 bp

Back to Erythronium

Pi 0.0238

MarkerSeek score 72.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 680
variable_sites: 49
pi: 0.0238
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTGCAACAAACAAGTAATAGA	AGCAGCGTGTCTACCAATTT	33.3	45.0	52.4	57.8	745-860 bp	1.0	49.1
rpl32-trnL-UAG_p2	GTGCAACAAACAAGTAATAGA	CCTAAGAGCAGCGTGTCTAC	33.3	55.0	52.4	57.8	751-866 bp	1.0	49.1
rpl32-trnL-UAG_p3	GTGCAACAAACAAGTAATAGA	CTAAGAGCAGCGTGTCTACC	33.3	55.0	52.4	57.8	750-865 bp	1.0	49.1
rpl32-trnL-UAG_p4	GTGCAACAAACAAGTAATAGA	TCCTAAGAGCAGCGTGTCTA	33.3	50.0	52.4	57.9	752-867 bp	1.0	49.0
rpl32-trnL-UAG_p5	GTGCAACAAACAAGTAATAGAA	TCCTAAGAGCAGCGTGTCTA	31.8	50.0	53.3	57.9	752-867 bp	1.0	49.0