Hotspot

rpl22-rps19

LSC · 82155-82213 · 59 bp

Back to Erythronium

Pi 0.0356

MarkerSeek score 61.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 5
pi: 0.0356
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTCCGCGAATCTGATCAAT	GCCTACTCTCACTTTCGCAA	50.0	50.0	58.5	57.9	227-234 bp	1.0	78.4
rpl22-rps19_p2	CGTCCGCGAATCTGATCAA	GCCTACTCTCACTTTCGCAA	52.6	50.0	58.0	57.9	227-234 bp	1.0	74.2
rpl22-rps19_p3	AACGTCCGCGAATCTGATC	GCCTACTCTCACTTTCGCAA	52.6	50.0	58.0	57.9	229-236 bp	1.0	74.1
rpl22-rps19_p4	GAACGTCCGCGAATCTGAT	GCCTACTCTCACTTTCGCAA	52.6	50.0	58.0	57.9	230-237 bp	1.0	74.1
rpl22-rps19_p5	CGTCCGCGAATCTGATCAAT	GTGCCTACTCTCACTTTCGC	50.0	55.0	58.5	58.6	229-236 bp	1.0	73.9