Hotspot

psaJ-rpl33

LSC · 65569-66176 · 608 bp

Back to Erythronium

Pi 0.0303

MarkerSeek score 71.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 47
pi: 0.0303
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	CGGGCACAACAACTAGTACA	50.0	50.0	58.1	57.8	694-744 bp	1.0	87.7
psaJ-rpl33_p2	ATGGTTTGGGTCTTTAGCGG	CGGGCACAACAACTAGTACA	50.0	50.0	58.2	57.8	699-749 bp	1.0	86.8
psaJ-rpl33_p3	TGGGTCTTTAGCGGGTCTAT	CGGGCACAACAACTAGTACA	50.0	50.0	57.8	57.8	693-743 bp	1.0	86.8
psaJ-rpl33_p4	TTGGGTCTTTAGCGGGTCTA	TTTCGGGCACAACAACTAGT	50.0	45.0	58.1	57.7	697-747 bp	1.0	86.2
psaJ-rpl33_p5	ATGGTTTGGGTCTTTAGCGG	TTTCGGGCACAACAACTAGT	50.0	45.0	58.2	57.7	702-752 bp	1.0	85.3