Hotspot

psbE-petL

LSC · 63037-64189 · 1153 bp

Back to Erythronium

Pi 0.0223

MarkerSeek score 74.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 75
pi: 0.0223
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCTGGGGTTATAGTTGAAGCA	50.0	47.6	57.9	58.0	1289-1317 bp	1.0	79.9
psbE-petL_p2	CAACCCGCAATGAATAGGGA	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1287-1315 bp	1.0	79.9
psbE-petL_p3	CAACCCGCAATGAATAGGGA	ACCAATCAATAGAGCTGGGG	50.0	50.0	57.9	57.0	1302-1330 bp	1.0	79.5
psbE-petL_p4	CAACCCGCAATGAATAGGGA	CCAATCAATAGAGCTGGGGT	50.0	50.0	57.9	57.0	1301-1329 bp	1.0	79.5
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGGGGTTATAGTTGAAGCAGC	50.0	47.6	57.9	58.0	1300-1328 bp	1.0	79.5