Hotspot

petA-psbJ

LSC · 61599-62257 · 659 bp

Back to Erythronium

Pi 0.0208

MarkerSeek score 58.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 38
pi: 0.0208
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAAGATCCATTACGCGTCCA	TAGGAGGCTTCCAATGACCA	50.0	50.0	58.1	58.0	1260-1333 bp	1.0	86.2
petA-psbJ_p2	CAAGATCCATTACGCGTCCA	TGGACTAGCTGTACCTACCG	50.0	55.0	58.1	58.0	1194-1267 bp	1.0	86.2
petA-psbJ_p3	CAAGATCCATTACGCGTCCA	GGACTAGCTGTACCTACCGT	50.0	55.0	58.1	58.0	1193-1266 bp	1.0	86.2
petA-psbJ_p4	CAAGATCCATTACGCGTCCA	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.1	58.0	1341-1414 bp	0.909091	82.5
petA-psbJ_p5	TATACCTCCAGGACCGGAAC	TAGGAGGCTTCCAATGACCA	55.0	50.0	57.9	58.0	1378-1425 bp	0.909091	82.2