Hotspot

pafII-cemA

LSC · 59154-59707 · 554 bp

Back to Erythronium

Pi 0.02

MarkerSeek score 72.00

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 554
variable_sites: 32
pi: 0.02
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	AAAAGCTGCCGAATTAGCCT	ATGAGAGAGAGACCCACCAG	45.0	55.0	58.2	57.8	657-685 bp	1.0	85.4
pafII-cemA_p2	AAAAGCTGCCGAATTAGCCT	GGGAGGGAAGTCAATGCTTT	45.0	50.0	58.2	57.8	610-638 bp	1.0	85.1
pafII-cemA_p3	CCTTGACTGGCACTGATGAG	ATGAGAGAGAGACCCACCAG	55.0	55.0	58.3	57.8	703-731 bp	1.0	84.3
pafII-cemA_p4	CCTTGACTGGCACTGATGAG	GGGAGGGAAGTCAATGCTTT	55.0	50.0	58.3	57.8	656-684 bp	1.0	84.0
pafII-cemA_p5	AAAAGCTGCCGAATTAGCCT	AATGAGAGAGAGACCCACCA	45.0	50.0	58.2	57.4	658-686 bp	1.0	81.6