Hotspot

ndhC-trnV-UAC

LSC · 48779-49761 · 983 bp

Back to Erythronium

Pi 0.0176
MarkerSeek score 70.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
983
variable_sites
50
pi
0.0176
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCCGGCCCTTCACTAATTG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1108-1168 bp 1.0 84.3
ndhC-trnV-UAC_p2 GCCCTTCACTAATTGGTGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1103-1163 bp 1.0 84.1
ndhC-trnV-UAC_p3 CTTTTCCGGCCCTTCACTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1111-1171 bp 1.0 83.7
ndhC-trnV-UAC_p4 TTCCGGCCCTTCACTAATTG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1107-1167 bp 1.0 81.6
ndhC-trnV-UAC_p5 GCCCTTCACTAATTGGTGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.2 57.5 1102-1162 bp 1.0 81.3