Hotspot

rpoB-trnC-GCA

LSC · 26167-27046 · 880 bp

Back to Erythronium

Pi 0.0174
MarkerSeek score 68.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
880
variable_sites
43
pi
0.0174
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCGTCAAGCCCTGATTAATGA GCGACACCCAGATTTGAACT 47.6 50.0 58.1 58.5 1001-1046 bp 1.0 75.1
rpoB-trnC-GCA_p2 TCCGTCAAGCCCTGATTAATG GCGACACCCAGATTTGAACT 47.6 50.0 58.1 58.5 1002-1047 bp 1.0 75.1
rpoB-trnC-GCA_p3 CCGTCAAGCCCTGATTAATGA TAAAGGATTTGCAGTCCCCC 47.6 50.0 58.1 57.5 976-1021 bp 1.0 74.8
rpoB-trnC-GCA_p4 TCCGTCAAGCCCTGATTAATG TAAAGGATTTGCAGTCCCCC 47.6 50.0 58.1 57.5 977-1022 bp 1.0 74.8
rpoB-trnC-GCA_p5 CCGTCAAGCCCTGATTAATGA CCTTACCACTTGGCCATGTC 47.6 55.0 58.1 58.5 955-1000 bp 1.0 74.8