Hotspot

trnS-GCU-trnG-UCC

LSC · 7888-8592 · 705 bp

Back to Erythronium

Pi 0.0216
MarkerSeek score 80.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
705
variable_sites
46
pi
0.0216
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AAGGGATAGCGGAGAGAGAG ACGAATCACACTTTTACCACT 55.0 38.1 57.7 55.5 836-864 bp 1.0 57.9
trnS-GCU-trnG-UCC_p2 TAGCGGAGAGAGAGGGATTC ACGAATCACACTTTTACCACT 55.0 38.1 57.7 55.5 830-858 bp 1.0 57.8
trnS-GCU-trnG-UCC_p3 CGGAGAGAGAGGGATTCGAA ACGAATCACACTTTTACCACT 55.0 38.1 58.3 55.5 827-855 bp 1.0 57.6
trnS-GCU-trnG-UCC_p4 TAGCGGAGAGAGAGGGATTC AGAACGAATCACACTTTTACCAC 55.0 39.1 57.7 57.3 833-861 bp 1.0 56.8
trnS-GCU-trnG-UCC_p5 TAGCGGAGAGAGAGGGATTC GAACGAATCACACTTTTACCACT 55.0 39.1 57.7 57.3 832-860 bp 1.0 56.8