Hotspot

rpl20

LSC · 67528-67911 · 384 bp

Back to Erythrodes

Pi 0.00781

MarkerSeek score 51.50

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 384
variable_sites: 3
pi: 0.00781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	GGAGTTCATTCTCCGGGAAA	CGGAGGAAAAGGATGACTGA	50.0	50.0	57.2	56.9	525 bp	1.0	65.7
rpl20_p2	GGAGTTCATTCTCCGGGAAA	CGGAGGAAAAGGATGACTGAG	50.0	52.4	57.2	58.1	525 bp	1.0	65.4
rpl20_p3	GGAGTTCATTCTCCGGGAAAT	CGGAGGAAAAGGATGACTGA	47.6	50.0	57.7	56.9	525 bp	1.0	61.7
rpl20_p4	GGAGTTCATTCTCCGGGAAAT	CGGAGGAAAAGGATGACTGAG	47.6	52.4	57.7	58.1	525 bp	1.0	61.4
rpl20_p5	TCTTCCCGGAGTTCATTCTC	CGGAGGAAAAGGATGACTGA	50.0	50.0	56.6	56.9	532 bp	1.0	61.2