Hotspot

atpF

LSC · 11387-12743 · 1357 bp

Back to Erythrodes

Pi 0.00886

MarkerSeek score 49.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1357
variable_sites: 12
pi: 0.00886
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CACTTGAAGTACGGTACCGG	ATTTATGGACTGGTCGTGGC	55.0	50.0	58.0	58.0	2016-2111 bp	1.0	84.4
atpF_p2	CCACTTGAAGTACGGTACCG	ATTTATGGACTGGTCGTGGC	55.0	50.0	58.0	58.0	2017-2112 bp	1.0	84.4
atpF_p3	CACTTGAAGTACGGTACCGG	TGACACGAGACCTCAGAACT	55.0	50.0	58.0	58.0	1752-1757 bp	1.0	80.6
atpF_p4	CCACTTGAAGTACGGTACCG	TGACACGAGACCTCAGAACT	55.0	50.0	58.0	58.0	1753-1758 bp	1.0	80.6
atpF_p5	CCCCTCCTTCCTGGAAAGTA	TGACACGAGACCTCAGAACT	55.0	50.0	58.0	58.0	2299-2304 bp	1.0	80.3