Hotspot

ndhH-rps15

SSC · 123061-123209 · 149 bp

Back to Erythrodes

Pi 0.0365
MarkerSeek score 65.00
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
149
variable_sites
5
pi
0.0365
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 TTTTGTAGCCGGTATAGTCA GATCAGTTAGATATTCGGGAACCA 40.0 41.7 53.8 57.9 194-206 bp 1.0 46.0
ndhH-rps15_p2 TTTTGTAGCCGGTATAGTCA TGGATCAGTTAGATATTCGGGAAC 40.0 41.7 53.8 57.9 196-208 bp 1.0 46.0
ndhH-rps15_p3 TTTTGTAGCCGGTATAGTCA TGGATCAGTTAGATATTCGGGA 40.0 40.9 53.8 55.9 196-208 bp 1.0 46.0
ndhH-rps15_p4 TTTTGTAGCCGGTATAGTCA ATCAGTTAGATATTCGGGAACCA 40.0 39.1 53.8 56.9 193-205 bp 1.0 46.0
ndhH-rps15_p5 TTTTTGTAGCCGGTATAGTCA GATCAGTTAGATATTCGGGAACCA 38.1 41.7 54.7 57.9 195-207 bp 1.0 46.0