Hotspot

psaC-ndhE

SSC · 117525-117848 · 324 bp

Back to Erythrodes

Pi 0.0216

MarkerSeek score 52.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 324
variable_sites: 7
pi: 0.0216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGACAAGCGGATTCACATCT	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.0	58.0	734 bp	1.0	80.7
psaC-ndhE_p2	GGACAAGCGGATTCACATCT	AAGCCGAAGCATGGTTAGAG	50.0	50.0	58.0	58.0	738 bp	1.0	80.6
psaC-ndhE_p3	AGCTCGAACACATTGGGTAC	CGAAGCATGGTTAGAGCACT	50.0	50.0	57.9	58.0	618 bp	1.0	80.3
psaC-ndhE_p4	AGCTCGAACACATTGGGTAC	AAGCCGAAGCATGGTTAGAG	50.0	50.0	57.9	58.0	622 bp	1.0	80.2
psaC-ndhE_p5	ACGGGCCTTTGTCAGTAAAG	CGAAGCATGGTTAGAGCACT	50.0	50.0	58.1	58.0	862 bp	0.5	59.0