Hotspot

rpl32-trnL-UAG

SSC · 113315-114192 · 878 bp

Back to Erythrodes

Pi 0.0139
MarkerSeek score 55.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
878
variable_sites
12
pi
0.0139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGACATAGAAGTGCGTTTTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.5 57.9 950-972 bp 1.0 44.7
rpl32-trnL-UAG_p2 TTGACATAGAAGTGCGTTTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.5 57.9 951-973 bp 1.0 44.7
rpl32-trnL-UAG_p3 TTTGACATAGAAGTGCGTTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.5 57.9 952-974 bp 1.0 44.7
rpl32-trnL-UAG_p4 TTTTGACATAGAAGTGCGTT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.5 57.9 953-975 bp 1.0 44.7
rpl32-trnL-UAG_p5 TTTTTGACATAGAAGTGCGT TCCTAAGAGCAGCGTGTCTA 35.0 50.0 53.5 57.9 954-976 bp 1.0 44.7