Hotspot

ndhF-rpl32

SSC · 112345-113140 · 796 bp

Back to Erythrodes

Pi 0.0176

MarkerSeek score 62.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 796
variable_sites: 14
pi: 0.0176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAGCGGAAAAAGGGGTATT	AGCGGCAGTAAAAGCTTTTTC	50.0	42.9	57.9	58.0	942-946 bp	1.0	72.0
ndhF-rpl32_p2	GGAGCGGAAAAAGGGGTATT	GCGGCAGTAAAAGCTTTTTCT	50.0	42.9	57.9	58.0	941-945 bp	1.0	72.0
ndhF-rpl32_p3	ACATAGTAACTGGGAGCGGA	AGCGGCAGTAAAAGCTTTTTC	50.0	42.9	57.8	58.0	954-958 bp	1.0	71.8
ndhF-rpl32_p4	ACATAGTAACTGGGAGCGGA	GCGGCAGTAAAAGCTTTTTCT	50.0	42.9	57.8	58.0	953-957 bp	1.0	71.8
ndhF-rpl32_p5	GGAGCGGAAAAAGGGGTATT	GCGAAATCTATTTCCACCGG	50.0	50.0	57.9	57.0	917-921 bp	1.0	71.8