Hotspot

ycf4-cemA

LSC · 59813-60535 · 723 bp

Back to Erythrodes

Pi 0.0153

MarkerSeek score 54.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 11
pi: 0.0153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TCGTCGCATCTTTCTTCGTT	CAACCATGGGTCGAGTGAAA	45.0	50.0	57.9	58.1	1446-1449 bp	1.0	79.7
ycf4-cemA_p2	TATGGGATCGCGGGTCTATT	CAACCATGGGTCGAGTGAAA	50.0	50.0	58.1	58.1	1580-1583 bp	1.0	79.6
ycf4-cemA_p3	ATCAGAATGGAGGCGAGAGA	CAACCATGGGTCGAGTGAAA	50.0	50.0	57.9	58.1	1403-1406 bp	1.0	79.6
ycf4-cemA_p4	TTTCGTTGGGGATTTCCTGG	CAACCATGGGTCGAGTGAAA	50.0	50.0	58.1	58.1	1472-1475 bp	1.0	79.5
ycf4-cemA_p5	TCGTCGCATCTTTCTTCGTT	GGTCGAGTGAAATCCGATCC	45.0	55.0	57.9	58.1	1438-1441 bp	1.0	79.4