Hotspot

ndhC-trnV-UAC

LSC · 49861-50445 · 585 bp

Back to Erythrodes

Pi 0.0157
MarkerSeek score 66.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
585
variable_sites
9
pi
0.0157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCGGAAATGACAAATGCCA AGAAGGTCTACGGTTCGAGT 45.0 50.0 58.1 57.8 705-759 bp 1.0 80.0
ndhC-trnV-UAC_p2 TCTGGTCCTTCCCTAATCGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 734-788 bp 1.0 78.9
ndhC-trnV-UAC_p3 AATCGGGGCTAAAATTCCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 720-774 bp 1.0 78.2
ndhC-trnV-UAC_p4 TCCGGAAATGACAAATGCCA AGCCCAATGCAACAAAATGC 45.0 45.0 58.1 58.5 787-841 bp 1.0 77.6
ndhC-trnV-UAC_p5 TCCGGAAATGACAAATGCCA GAAGGTCTACGGTTCGAGTC 45.0 55.0 58.1 57.5 704-758 bp 1.0 77.3