Hotspot

ndhJ-ndhK

LSC · 48639-48763 · 125 bp

Back to Erythrodes

Pi 0.032

MarkerSeek score 70.90

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 4
pi: 0.032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GAGTCCCAATCCTCGGTTTT	ACTGGAAATTACGATCAGGGA	50.0	42.9	57.5	56.4	352 bp	1.0	56.5
ndhJ-ndhK_p2	AGTCCCAATCCTCGGTTTTT	ACTGGAAATTACGATCAGGGA	45.0	42.9	57.0	56.4	351 bp	1.0	52.6
ndhJ-ndhK_p3	GAGTCCCAATCCTCGGTTTT	ACTGGAAATTACGATCAGGGAT	50.0	40.9	57.5	57.0	352 bp	1.0	52.6
ndhJ-ndhK_p4	GAGTCCCAATCCTCGGTTTT	ACTGGAAATTACGATCAGGG	50.0	45.0	57.5	54.8	352 bp	1.0	51.7
ndhJ-ndhK_p5	GAGTCCCAATCCTCGGTTTTT	ACTGGAAATTACGATCAGGGA	47.6	42.9	58.2	56.4	352 bp	1.0	50.7