Hotspot

trnF-GAA-ndhJ

LSC · 47606-48161 · 556 bp

Back to Erythrodes

Pi 0.0055

MarkerSeek score 62.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 3
pi: 0.0055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	TACTGGTCGGGATAGCTCAG	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	710-726 bp	1.0	80.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	666-682 bp	1.0	80.2
trnF-GAA-ndhJ_p3	TACTGGTCGGGATAGCTCAG	ATAGGCTGGCCCTTACGTAA	55.0	50.0	58.0	58.2	700-716 bp	1.0	79.8
trnF-GAA-ndhJ_p4	TGCTAGGATGATGCAGGGTA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.9	57.9	728-744 bp	1.0	79.7
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	656-672 bp	1.0	79.7