Hotspot

trnE-UUC-trnT-GGU

LSC · 30710-31125 · 416 bp

Back to Erythrodes

Pi 0.0145

MarkerSeek score 58.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 416
variable_sites: 6
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	519-520 bp	1.0	79.7
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	498-499 bp	1.0	79.3
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	495-496 bp	1.0	79.1
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	497-498 bp	1.0	78.8
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	TGGAAAAAGCCCCTTATCGG	50.0	50.0	57.8	57.9	543-544 bp	1.0	78.6