Hotspot

rpoB-trnC-GCA

LSC · 26379-27556 · 1178 bp

Back to Erythrodes

Pi 0.00509

MarkerSeek score 61.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1178
variable_sites: 6
pi: 0.00509
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCCGATTAATGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	57.5	1318 bp	1.0	75.7
rpoB-trnC-GCA_p2	CGTCAAGCCCCGATTAATGA	CGACACCCAGATTCGAACTG	50.0	55.0	58.1	58.6	1342 bp	1.0	74.7
rpoB-trnC-GCA_p3	CGTCAAGCCCCGATTAATGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.1	58.8	1355 bp	1.0	73.8
rpoB-trnC-GCA_p4	AAATTCTTCCGTCAAGCCCC	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	57.5	1327 bp	1.0	72.5
rpoB-trnC-GCA_p5	CGTCAAGCCCCGATTAATGA	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.1	58.0	1319 bp	1.0	71.7