Hotspot

ycf1

SSC · 121415-126697 · 5283 bp

Back to Erythrina

Pi 0.0131

MarkerSeek score 48.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5283
variable_sites: 103
pi: 0.0131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAACGGCTTCCATTATGGGT	GCAGAATACTCCGATCGACC	45.0	55.0	57.5	57.9	869-881 bp	1.0	78.5
ycf1_p2	AAACGGCTTCCATTATGGGT	AACTCCCCCAGGTTTAAGGA	45.0	50.0	57.5	57.9	540 bp	1.0	77.2
ycf1_p3	AAACGGCTTCCATTATGGGT	ACTCCCCCAGGTTTAAGGAA	45.0	50.0	57.5	57.9	539 bp	1.0	77.2
ycf1_p4	AAACGGCTTCCATTATGGGT	TATGTAGGATCGGGCAGTGA	45.0	50.0	57.5	57.6	797-809 bp	1.0	76.1
ycf1_p5	AAACGGCTTCCATTATGGGT	GGATCGGGCAGTGAAAAGAA	45.0	50.0	57.5	58.2	803 bp	0.666667	62.6