Hotspot

rpoC2

LSC · 40396-44499 · 4104 bp

Back to Erythrina

Pi 0.0102

MarkerSeek score 46.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4104
variable_sites: 62
pi: 0.0102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGAGTCGGATCGGACTTACA	GCCAAATAAGGGTTAGCCGA	50.0	50.0	57.9	58.0	621-636 bp	1.0	83.7
rpoC2_p2	TGAGTCGGATCGGACTTACA	TCGCCAAATAAGGGTTAGCC	50.0	50.0	57.9	58.0	623-638 bp	1.0	83.7
rpoC2_p3	TGAGTCGGATCGGACTTACA	TTCGGCTCGCAATAATCCAA	50.0	45.0	57.9	58.0	1060-1075 bp	1.0	81.9
rpoC2_p4	TGAGTCGGATCGGACTTACA	TTAGTTCACCGGGCGAAAAA	50.0	45.0	57.9	58.0	1040-1055 bp	1.0	81.9
rpoC2_p5	TGAGTCGGATCGGACTTACA	ATCCAATTAGTTCACCGGGC	50.0	50.0	57.9	57.9	1046-1061 bp	1.0	81.7