Hotspot

rpoC1

LSC · 37340-40203 · 2864 bp

Back to Erythrina

Pi 0.00828

MarkerSeek score 62.40

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2864
variable_sites: 35
pi: 0.00828
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCGGATTCATAGGAAGGAAGC	TCGCGAAGACCTGAATTTACA	47.6	42.9	57.2	57.7	2950-2978 bp	1.0	57.4
rpoC1_p2	CGGATTCATAGGAAGGAAGCT	TCGCGAAGACCTGAATTTACA	47.6	42.9	57.2	57.7	2949-2977 bp	1.0	57.3
rpoC1_p3	TCGGATTCATAGGAAGGAAGC	CGCGAAGACCTGAATTTACAC	47.6	47.6	57.2	57.6	2949-2977 bp	1.0	56.8
rpoC1_p4	CGGATTCATAGGAAGGAAGCT	CGCGAAGACCTGAATTTACAC	47.6	47.6	57.2	57.6	2948-2976 bp	1.0	56.8
rpoC1_p5	TCGGATTCATAGGAAGGAAGC	AGTTAAATCGCGAAGACCTGA	47.6	42.9	57.2	57.4	2957-2985 bp	1.0	55.1