Hotspot

rpl16

LSC · 79300-80866 · 1567 bp

Back to Erythrina

Pi 0.0141
MarkerSeek score 58.30
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1567
variable_sites
32
pi
0.0141
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCGTCCTAAAGACCTCTTTCCT TCATTTCATATTGTTGATAGTATTGCT 45.5 25.9 58.2 55.8 1630-1643 bp 1.0 42.7
rpl16_p2 TCGTCCTAAAGACCTCTTTCC TCATTTCATATTGTTGATAGTATTGCT 47.6 25.9 56.8 55.8 1630-1643 bp 1.0 42.7
rpl16_p3 CGTCCTAAAGACCTCTTTCCT TCATTTCATATTGTTGATAGTATTGCT 47.6 25.9 56.8 55.8 1629-1642 bp 1.0 42.7
rpl16_p4 TCGTCCTAAAGACCTCTTTCCT TTTCATATTGTTGATAGTATTGCT 45.5 25.0 58.2 52.6 1627-1640 bp 1.0 42.7
rpl16_p5 TCGTCCTAAAGACCTCTTTCC TTTCATATTGTTGATAGTATTGCT 47.6 25.0 56.8 52.6 1627-1640 bp 1.0 42.7