Hotspot

psaJ-rpl33

LSC · 64578-65117 · 540 bp

Back to Erythrina

Pi 0.0154

MarkerSeek score 66.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 540
variable_sites: 12
pi: 0.0154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CATATCTTTCCGTGGCACCA	CACAACCCGTACACTCCAAA	50.0	50.0	58.0	58.0	680-708 bp	1.0	84.7
psaJ-rpl33_p2	ACATATCTTTCCGTGGCACC	CACAACCCGTACACTCCAAA	50.0	50.0	58.0	58.0	681-709 bp	1.0	84.7
psaJ-rpl33_p3	TTGGCGGGTCTCTTGATAGA	CACAACCCGTACACTCCAAA	50.0	50.0	58.1	58.0	633-661 bp	1.0	84.0
psaJ-rpl33_p4	TACTCTATGGTTCGGGGCTT	CACAACCCGTACACTCCAAA	50.0	50.0	57.8	58.0	652-680 bp	1.0	83.7
psaJ-rpl33_p5	CTCTATGGTTCGGGGCTTTG	CACAACCCGTACACTCCAAA	55.0	50.0	58.3	58.0	650-678 bp	1.0	82.5