Hotspot

psbK-trnQ-UUG

LSC · 53783-54219 · 437 bp

Back to Erythrina

Pi 0.0276

MarkerSeek score 71.90

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 437
variable_sites: 17
pi: 0.0276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	ATAGGCTTCGGGCAATTTCG	TACTCTGGGACGGAAGGATT	50.0	50.0	58.7	57.5	597-603 bp	1.0	73.2
psbK-trnQ-UUG_p2	CATAGGCTTCGGGCAATTTC	TACTCTGGGACGGAAGGATT	50.0	50.0	57.2	57.5	598-604 bp	1.0	72.4
psbK-trnQ-UUG_p3	ATAGGCTTCGGGCAATTTCG	GACCAAAACCCGTTGCCTTA	50.0	50.0	58.7	58.7	559-565 bp	1.0	72.3
psbK-trnQ-UUG_p4	CATAGGCTTCGGGCAATTTC	GACCAAAACCCGTTGCCTTA	50.0	50.0	57.2	58.7	560-566 bp	1.0	71.6
psbK-trnQ-UUG_p5	ATAGGCTTCGGGCAATTTCG	ATACTCTGGGACGGAAGGAT	50.0	50.0	58.7	57.2	598-604 bp	1.0	71.3