Hotspot

psbD-trnT-GGU

LSC · 27958-29207 · 1250 bp

Back to Erythrina

Pi 0.0191

MarkerSeek score 60.40

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1250
variable_sites: 32
pi: 0.0191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AATTTACCAAGAGCTATAGTCA	GTAACGCCATGGTAAGACGT	31.8	50.0	52.3	58.0	943-1325 bp	1.0	46.1
psbD-trnT-GGU_p2	AATTTACCAAGAGCTATAGTCA	GTGGTAGAGTAACGCCATGG	31.8	55.0	52.3	58.1	951-1333 bp	1.0	46.1
psbD-trnT-GGU_p3	AATTTACCAAGAGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	31.8	50.0	52.3	58.1	954-1336 bp	1.0	46.1
psbD-trnT-GGU_p4	AAATTTACCAAGAGCTATAGTCA	GTAACGCCATGGTAAGACGT	30.4	50.0	53.2	58.0	944-1326 bp	1.0	46.1
psbD-trnT-GGU_p5	AAATTTACCAAGAGCTATAGTCA	GTGGTAGAGTAACGCCATGG	30.4	55.0	53.2	58.1	952-1334 bp	1.0	46.1