Hotspot

trnS-UGA-psbC

LSC · 25320-25526 · 207 bp

Back to Erythrina

Pi 0.0322

MarkerSeek score 66.30

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 207
variable_sites: 10
pi: 0.0322
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	CGCCGGATTTGAAAAAGGAA	55.0	45.0	58.0	57.3	366-370 bp	1.0	76.5
trnS-UGA-psbC_p2	ATAGCCCCGGTCTTGAAAAC	CGCCGGATTTGAAAAAGGAA	50.0	45.0	57.9	57.3	346-350 bp	1.0	75.8
trnS-UGA-psbC_p3	GCTTTTGGAGAGATGGCTGA	CGCCGGATTTGAAAAAGGAA	50.0	45.0	57.9	57.3	373-377 bp	1.0	75.7
trnS-UGA-psbC_p4	AGCTTTTGGAGAGATGGCTG	CGCCGGATTTGAAAAAGGAA	50.0	45.0	57.9	57.3	374-378 bp	1.0	75.6
trnS-UGA-psbC_p5	GGGTTCGAATCCCTCTCTCT	CGCCGGATTTGAAAAAGGAA	55.0	45.0	58.2	57.3	297-301 bp	1.0	75.5