Hotspot

ycf3-psaA

LSC · 18114-18852 · 739 bp

Back to Erythrina

Pi 0.0126

MarkerSeek score 68.80

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 14
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AGCCCTTACCCCTGGTAATT	ATGACCAGGTTTTGCCCATT	50.0	45.0	58.0	58.0	888-899 bp	1.0	82.2
ycf3-psaA_p2	AGCCCTTACCCCTGGTAATT	AATGACCAGGTTTTGCCCAT	50.0	45.0	58.0	58.0	889-900 bp	1.0	82.1
ycf3-psaA_p3	AGCTATAGCCCTTACCCCTG	AATGACCAGGTTTTGCCCAT	55.0	45.0	58.0	58.0	895-906 bp	1.0	81.9
ycf3-psaA_p4	AGCTATAGCCCTTACCCCTG	ATGACCAGGTTTTGCCCATT	55.0	45.0	58.0	58.0	894-905 bp	1.0	81.9
ycf3-psaA_p5	AGCCCTTACCCCTGGTAATT	TGCCCATTCCTCGAAAGAAG	50.0	50.0	58.0	57.9	876-887 bp	1.0	81.5