Hotspot

ndhK-ndhJ

LSC · 11605-11718 · 114 bp

Back to Erythrina

Pi 0.076

MarkerSeek score 81.70

Primer available yes

Label ndhK-ndhJ

Feature Metadata

label_name: ndhK-ndhJ
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 13
pi: 0.076
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhJ_p1	ACCGCCATCTACTTCAGAGA	AGAAGACAAACGACCTTGCA	50.0	45.0	57.9	57.7	178-198 bp	1.0	81.9
ndhK-ndhJ_p2	ACCGCCATCTACTTCAGAGA	AGCCAAGAAGACAAACGACC	50.0	50.0	57.9	58.4	183-203 bp	1.0	81.1
ndhK-ndhJ_p3	ACCGCCATCTACTTCAGAGA	GCCAAGAAGACAAACGACCT	50.0	50.0	57.9	58.4	182-202 bp	1.0	81.1
ndhK-ndhJ_p4	GATGCAGTACTCATACCGGC	AGAAGACAAACGACCTTGCA	55.0	45.0	58.2	57.7	224-244 bp	1.0	80.2
ndhK-ndhJ_p5	GCAGTACTCATACCGGCAAT	AGAAGACAAACGACCTTGCA	50.0	45.0	57.8	57.7	221-241 bp	1.0	80.0