Hotspot

ycf1

SSC · 122855-128440 · 5586 bp

Back to Erythranthe

Pi 0.0214

MarkerSeek score 63.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5586
variable_sites: 454
pi: 0.0214
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCTTTTCCATCACTTTGGC	GCAGAATACCGTCACCCATT	50.0	50.0	57.9	58.0	320-557 bp	1.0	91.0
ycf1_p2	GGTCTATTCCAGCGCTTTGA	GCAGAATACCGTCACCCATT	50.0	50.0	58.0	58.0	454-463 bp	0.882353	83.2
ycf1_p3	GGTCTATTCCAGCGCTTTGA	GGGCCCTCTTATCTCTTCCT	50.0	55.0	58.0	57.9	1046-1055 bp	0.882353	78.2
ycf1_p4	GGTCTATTCCAGCGCTTTGA	ACCGTCACCCATTTTGACTC	50.0	50.0	58.0	58.1	447-456 bp	0.705882	70.4
ycf1_p5	GGTCTATTCCAGCGCTTTGA	CCGTCACCCATTTTGACTCA	50.0	50.0	58.0	58.1	446-455 bp	0.705882	70.4