Hotspot

ndhF

SSC · 109813-112056 · 2244 bp

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Pi 0.0154

MarkerSeek score 46.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 128
pi: 0.0154
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGGATGCGGCAAAATCATTC	GCCCAATCTCCCTTCCTTTT	45.0	50.0	57.4	57.8	2999 bp	0.058824	35.8
ndhF_p2	CTGGATGCGGCAAAATCATT	GCCCAATCTCCCTTCCTTTT	45.0	50.0	57.4	57.8	3000 bp	0.058824	35.7
ndhF_p3	GGATGCGGCAAAATCATTCT	GCCCAATCTCCCTTCCTTTT	45.0	50.0	57.1	57.8	2998 bp	0.058824	33.4
ndhF_p4	TCTGGATGCGGCAAAATCAT	CCCAATCTCCCTTCCTTTTCC	45.0	52.4	58.2	58.3	3000 bp	0.058824	30.4
ndhF_p5	TGGATGCGGCAAAATCATTC	TGCCCAATCTCCCTTCCTTT	45.0	50.0	57.4	58.9	3000 bp	0.058824	30.4